产品详情
  • 产品名称: HTB-9 5637 人膀胱癌细胞

  • 产品型号: HTB-9
  • 产品厂商:其它品牌
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
简单介绍 HTB-9 5637 人膀胱癌细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和**培养条件!
详情介绍:

 

HTB-9 5637 人膀胱癌细胞

ATCC® Number:  HTB-9™       
Designations:  5637 
Depositors:   G Cannon 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens (human) 
Morphology: epithelial

 HTB-9 5637 人膀胱癌细胞
Source: Organ: urinary bladder
Tumor Stage: grade II
Disease: carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Isolation:  Isolation date: March, 1974
Applications: transfection host (Roche FuGENE® Transfection Reagents)
Tumorigenic: Yes 
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11
D13S317: 11
D16S539: 9
D5S818: 11,12
D7S820: 10,11
THO1: 7,9
TPOX: 8,9
vWA: 16,18
Cytogenetic Analysis: modal chromosome number = 67; range = 59 to 71The stemline modal chromosome number is 67 occurring at 36%, and polyploidy at 0.6%. Fourteen marker chromosomes were common to these cells: 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q) and 8 others of unknown origin. Additional markers: der(5)t(5;7)(q31;p11) and 1p were found that were specific to a minor subpopulation. Microchromosomes (larger than double minutes) and double minutes (DM) were also found in a small population of cells. Normal N13 was absent; and N21 was found only in the minor subpopulation.One, or occasionally two, Y chromosomes were present in each cell.
Isoenzymes:  AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 2 HTB-9 5637 人膀胱癌细胞
Age:  68 years ***** 
Gender:  male 
Ethnicity:  Caucasian 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:  Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5% HTB-9 5637 人膀胱癌细胞
 



沪公网安备 31010902002433号