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  • 产品名称: A-673 人横纹肌瘤细胞

  • 产品型号: CRL-1598
  • 产品厂商:其它品牌
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简单介绍:
简单介绍 A-673 人横纹肌瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;胞库管理规范,提供的细胞株背景清楚,提供参考文献和**培养条件!
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A-673 人横纹肌瘤细胞 的详细介绍

A-673 人横纹肌瘤细胞

ATCC® Number:  CRL-1598™     

Designations:  A-673 
Depositors:   SA Aaronson 
Biosafety Level: 1 
Shipped:  frozen 
Medium & Serum:  See Propagation 
Growth Properties: adherent
Organism: Homo sapiens (human) 
Morphology: polygonal

 A-673 人横纹肌瘤细胞
Source: Tissue: muscle
Disease: rhabdomyosarcoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 
 
Tumorigenic: Yes 
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 8,13
D16S539: 11
D5S818: 11,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 15,18
Age:  15 years 
Gender:  female 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C A-673 人横纹肌瘤细胞
Subculturing:  Protocol: Remove medium, rinse monolayer with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:20 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758 
 A-673 人横纹肌瘤细胞
 

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