产品名称：Nexterion Slide H – 3D Hydrogel Coated Nexterion Slide H - 3D水凝胶涂层

Cleanroom cleaned and packaged

Sold in packs of 25 slides

Laser Barcoded

Please inquire for volume pricing

Slide dimensions:75.6 x 25.0 x 1.0 mm

SKU: 1070936

Categories: Coated Slides, Uncoated Coverslips

洁净室清洁和包装

以25张幻灯片包装出售

激光条形码

请咨询批量价格

滑动尺寸：75.6 x 25.0 x 1.0 mm

SKU：1070936

分类：涂层幻灯片，无涂层盖玻片

Description

SCHOTT launched NEXTERION® Slide H as a dedicated slide surface for printing protein microarrays, as it is ideally suited for the covalent immobilization of peptides and proteins such as antibodies, antibody fragments, enzymes, or receptors. For many protein microarray applications NEXTERION® Slide H has proven to be a very attractive alternative to the commonly used nitrocellulose coated slide, especially where low background, or slide transparency are important considerations. Since its introduction, the slide coating has also been successfully used with amino-modified oligonucleotides, and has become the slide of choice for printing amino-linked glycan microarrays. Carbohydrate arrays are a rapidly growing area of microarray research and NEXTERION® Slide H is an excellent choice for use in the rapid screening of carbohydrateprotein interactions. The permeable, polymer coating has a large immobilization capacity, and helps to preserve the native three-dimensional structure of complex bio-molecules, thus maintaining conformation and functionality. NEXTERION® Slide H produces excellent signal-to-background ratios and an exceptionally wide dynamic range compared to conventional “two-dimensional” coatings through a unique combination of low non-specific binding characteristics, and high probe loading capacity. Even very low intensity signals, such as those obtained from low-abundance analytes, or weakly expressed genes can be reliably detected and quantified on NEXTERION® Slide H. The robust coating matrix is fully compatible with commercial microarray printers and scanners. Simple and robust protocols are available making NEXTERION® Slide H easy-to-use.

Highly reproducible coating

NEXTERION® Slide H is fabricated using a proprietary thin-film deposition process optimized by SCHOTT to produce a uniform and reproducible polymer coating on one side of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the presence of particles before and after coating. The surface is applied in a tightly controlled, class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence.

Excellent spot morphology and signal-to-background ratios

NEXTERION® Slide H provides excellent spot morphologies and reproducible spot sizes over a wide range of probe concentrations for protein, oligonucleotide and other bio-molecule microarray applications.

Spot morphology NEXTERION hydrogel coated microarray slides

Figure shows scanned images of NEXTERION® Slide H when evaluated against the market leading threedimensional competitor slide in an anti-IgG/IgG interaction study using a range of probe concentrations.

Signal-to-Background ration NEXTERION Slide H

NEXTERION® Slide H

Signal-to-background-ratio of NEXTERION hydrogel coated slides

Leading 3D slide

Epoxysilane slide

Figure illustrates the superior signal-to-background ratios on NEXTERION® Slide H when compared to the market leading three-dimensional slide, and a conventional two-dimensional coating. This was demonstrated in two-color hybridization experiments following the recommended protocols, using amino-modified oligonucleotide probes.

Packaging and storage

NEXTERION® Slide H are packaged in chemically stable plastic boxes and sealed under an inert atmosphere. The slides are ready-to-use from the box, and are stable for 12 months in the sealed packaging when stored at – 20 ºC.

描述

肖特推出NEXTERION®SlideH作为印刷蛋白质微阵列的专用载玻片表面，因为它非常适用于肽和蛋白质（如抗体，抗体片段，酶或受体）的共价固定。对于许多蛋白质微阵列应用，NEXTERION®SlideH已被证明是常用的硝化纤维素涂层载玻片的一种非常有吸引力的替代品，特别是在低背景或载玻片透明度是重要考虑因素的情况下。自推出以来，载玻片涂层也成功地与氨基修饰的寡核苷酸一起使用，并且已成为印刷氨基连接的聚糖微阵列的优选载玻片。碳水化合物阵列是微阵列研究的快速增长领域，NEXTERION®SlideH是用于快速筛选碳水化合物蛋白质相互作用的优良选择。可渗透的聚合物涂层具有大的固定能力，并有助于保持复杂生物分子的天然三维结构，从而保持构象和功能。 NEXTERION®SlideH通过独特的低非特异性结合特性和高探针负载能力，与传统的“二维”涂层相比，具有出色的信号背景比和*宽的动态范围。即使是非常低强度的信号，例如从低丰度分析物获得的信号，或弱表达的基因，也可以在NEXTERION®SlideH上可靠地检测和定量。坚固的涂层基质与商业微阵列打印机和扫描仪完全兼容。 NEXTERION®SlideH易于使用，提供简单而强大的协议。

高度可重复的涂层

NEXTERION®SlideH采用肖特优化的专有薄膜沉积工艺制造，可在高质量硼硅酸盐玻璃载玻片的一侧产生均匀且可重复的聚合物涂层。在涂覆之前和之后，分别检查所有载玻片的物理缺陷和颗粒的存在。表面应用于严格控制的100级洁净室设施中，使涂层载玻片具有高度均匀的表面特性和低自发荧光。

优异的斑点形态和信号与背景比

NEXTERION®SlideH在蛋白质，寡核苷酸和其他生物分子微阵列应用的**探针浓度范围内提供出色的斑点形态和可重现的斑点大小。

斑点形态NEXTERION水凝胶涂覆的微阵列载玻片

图中显示了NEXTERION®SlideH的扫描图像，当使用一系列探针浓度进行抗IgG / IgG相互作用研究时，针对市场优越的三维竞争者载玻片进行评估。

信号到背景比率NEXTERION幻灯片H.

NEXTERION®SlideH

NEXTERION水凝胶涂层载玻片的信号与背景比

优越的3D幻灯片

环氧硅烷滑动

图中显示了与市场优越的三维载玻片和传统的二维涂层相比，NEXTERION®SlideH的优异信号与背景比。使用氨基修饰的寡核苷酸探针，在**的方案之后的双色杂交实验中证明了这一点。

包装和储存

NEXTERION®SlideH采用化学稳定的塑料盒包装，在惰性气氛下密封。这些载玻片可以从盒子中随时使用，在-20℃下储存时，可以在密封包装中稳定12个月。